Preparation of Cytolysin A (ClyA) Nanopores

Nicole Stéphanie Galenkamp, Veerle Van Meervelt, Natalie Lisa Mutter, Nieck Jordy van der Heide, Carsten Wloka, Giovanni Maglia*

*Bijbehorende auteur voor dit werk

Onderzoeksoutput: ChapterAcademicpeer review

2 Citaten (Scopus)

Samenvatting

The ionic currents passing through nanopores can be used to sequence DNA and identify molecules at the single-molecule level. Recently, researchers have started using nanopores for the detection and analysis of proteins, providing a new platform for single-molecule enzymology studies and more efficient biomolecular sensing applications. For this approach, the homo-oligomeric Cytolysin A (ClyA) nanopore has been demonstrated as a powerful tool. Here, we describe a simple protocol allowing the production of ClyA nanopores. Monomers of ClyA are expressed in Escherichia coli and oligomerized in the presence of detergent. Subsequently, different oligomer variants are electrophoretically resolved and stored in a gel matrix for long-term use.

Originele taal-2English
TitelNanopore Technology
SubtitelMethods in Molecular Biology
RedacteurenM.A. Fahie
Plaats van productieNew York
UitgeverijHumana Press
Pagina's11-18
Aantal pagina's8
ISBN van elektronische versie978-1-0716-0806-7
ISBN van geprinte versie978-1-0716-0805-0
DOI's
StatusPublished - 2021

Publicatie series

NaamMethods in Molecular Biology
Volume2186
ISSN van geprinte versie1064-3745

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