Presence of collagen IV in the ciliary zonules of the human eye: An immunohistochemical study by LM and TEM

LI Los*, RJ van der Worp, MJA van Luyn, JMM Hooymans

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

11 Citaten (Scopus)


The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission electron microscopic (LM and TEM) study identified collagen IV as a novel component of this coating. Collagen IV was demonstrated by pre-embedding and postembedding immunohistochemical (IHC) techniques using monoclonal and polyclonal antibodies. The specificity of the polyclonal anticollagen IV antibody was verified by ELISA.

Originele taal-2English
Pagina's (van-tot)789-795
Aantal pagina's7
TijdschriftJournal of Histochemistry & Cytochemistry
Nummer van het tijdschrift6
StatusPublished - jun.-2004

Citeer dit