Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium

Y.H. Lu, J.C. Knol, M.H.K. Linskens, K. Friehs, P. J. M. van Haastert, E. Flaschel

OnderzoeksoutputAcademicpeer review

18 Citaten (Scopus)
34 Downloads (Pure)


Human Fas ligand (hFasL) is of considerable interest since it is a type II transmembrane glycoprotein that induces programmed cell death, or apoptosis. In this study Dictyostelium discoideum was used to produce a soluble form of the human Fas ligand. The recombinant cells were adapted to a modified synthetic FM medium, called SIH medium. Cells adapted to the SIH medium reached about 2 times higher cell densities and hFasL concentrations on this medium compared with cells growing on the standard complex medium HL-5C. Even higher values were achieved by a dissolved oxygen-controlled fed-batch cultivation in a conventional stirred bioreactor on SIH medium. Cell densities of up to 5.5×107 ml−1 and a maximum hFasL concentration of 148 μg l−1 were obtained. These results were further improved by means of continuous cultivation of D. discoideum in a bioreactor equipped with cell retention by microfiltration. At low space velocity very high cell densities of up to 2.4×108 ml−1 and hFasL concentrations of up to 205 μg l−1 were achieved.
Originele taal-2English
Pagina's (van-tot)243-251
Aantal pagina's9
TijdschriftJournal of Biotechnology
Nummer van het tijdschrift3
StatusPublished - 18-mrt-2004



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