Proliferation-promoting roles of linear and circular PVT1 are independent of their ability to bind miRNAs in B-cell lymphoma

Xing Zhao, Anke van den Berg*, Melanie Winkle, Jasper Koerts, Annika Seitz, Debora de Jong, Bea Rutgers, Tineke van der Sluis, Emke Bakker, Joost Kluiver*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

16 Downloads (Pure)

Samenvatting

Plasmacytoma Variant Translocation 1 (PVT1) is a long non-coding RNA located at 8q24.21 immediately downstream of MYC. Both the linear and circular PVT1 transcripts contribute to cancer pathogenesis by binding microRNAs. However, little is known about their roles in B-cell lymphoma. Here we studied their expression patterns, role in growth, and ability to bind miRNAs in B-cell lymphoma. Linear PVT1 transcripts were downregulated in B-cell cell lymphoma lines compared to germinal center B cells, while circPVT1 levels were increased. Two Hodgkin lymphoma cell lines had a homozygous deletion including the 5' region of the PVT1 locus, resulting in a complete lack of circPVT1 and 5' linear PVT1 transcripts. Inhibition of both linear and circular PVT1 decreased growth of Burkitt lymphoma, while the effects on Hodgkin lymphoma and diffuse large B cell lymphoma were less pronounced. Overexpression of circPVT1 promoted growth of B-cell lymphoma lacking or having low endogenous circPVT1 levels. Contrary to other types of cancer, linear and circular PVT1 transcripts did not interact with miRNAs in B-cell lymphoma. Overall, we showed an opposite expression pattern of linear and circular PVT1 in B-cell lymphoma. Their effect on growth was independent of their ability to bind miRNAs.

Originele taal-2English
Artikelnummer126744
Aantal pagina's10
TijdschriftInternational Journal of Biological Macromolecules
Volume253
Nummer van het tijdschrift2
Vroegere onlinedatum7-sep.-2023
DOI's
StatusPublished - 31-dec.-2023

Citeer dit