Prolonged cigarette smoke exposure alters mitochondrial structure and function in airway epithelial cells

Roland F. Hoffmann*, Sina Zarrintan, Simone M. Brandenburg, Arjan Kol, Harold G. de Bruin, Shabnam Jafari, Freark Dijk, Dharamdajal Kalicharan, Marco Kelders, Harry R. Gosker, Nick H. T. ten Hacken, Johannes J. van der Want, Antoon J. M. van Oosterhout, Irene H. Heijink

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

173 Citaten (Scopus)
231 Downloads (Pure)

Samenvatting

Background: Cigarette smoking is the major risk factor for COPD, leading to chronic airway inflammation. We hypothesized that cigarette smoke induces structural and functional changes of airway epithelial mitochondria, with important implications for lung inflammation and COPD pathogenesis.

Methods: We studied changes in mitochondrial morphology and in expression of markers for mitochondrial capacity, damage/biogenesis and fission/fusion in the human bronchial epithelial cell line BEAS-2B upon 6-months from ex-smoking COPD GOLD stage IV patients to age-matched smoking and never-smoking controls.

Results: We observed that long-term CSE exposure induces robust changes in mitochondrial structure, including fragmentation, branching and quantity of cristae. The majority of these changes were persistent upon CSE depletion. Furthermore, long-term CSE exposure significantly increased the expression of specific fission/fusion markers (Fis1, Mfn1, Mfn2, Drp1 and Opa1), oxidative phosphorylation (OXPHOS) proteins (Complex II, III and V), and oxidative stress (Mn-SOD) markers. These changes were accompanied by increased levels of the pro-inflammatory mediators IL-6, IL-8, and IL-1 beta. Importantly, COPD primary bronchial epithelial cells (PBECs) displayed similar changes in mitochondrial morphology as observed in long-term CSE-exposure BEAS-2B cells. Moreover, expression of specific OXPHOS proteins was higher in PBECs from COPD patients than control smokers, as was the expression of mitochondrial stress marker PINK1.

Conclusion: The observed mitochondrial changes in COPD epithelium are potentially the consequence of long-term exposure to cigarette smoke, leading to impaired mitochondrial function and may play a role in the pathogenesis of COPD.

Originele taal-2English
Artikelnummer97
Aantal pagina's12
TijdschriftRespiratory Research
Volume14
DOI's
StatusPublished - 2-okt-2013

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