Protective effects of lactic acid bacteria on gut epithelial barrier dysfunction are Toll like receptor 2 and protein kinase C dependent

Chengcheng Ren*, Qiuxiang Zhang, Bart J de Haan, Marijke M Faas, Hao Zhang, Paul de Vos

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    13 Citaten (Scopus)
    43 Downloads (Pure)

    Samenvatting

    Lactic acid bacteria (LAB) are recognized for support of host gut homeostasis but the precise mechanisms remain to be identified. LABs interact with Toll-like receptors (TLRs) which might stimulate barrier function of gut epithelial cells. We previously identified six TLR2-signalling LAB strains. As TLR2 is involved in barrier-function enhancement in gut-epithelium, the epithelial barrier-protective effect of these TLR2-signalling strains was studied by using T84 human colorectal cancer cell monolayer as an in vitro gut epithelial barrier model. The protein kinase C (PKC) dependent barrier disruptor A23187 and mitogen-activated protein kinase dependent barrier stressor deoxynivalenol were tested to determine which pathways LAB influenced. We found that exclusively the PKC dependent disruption was prevented by the selected TLR2-signalling LAB strains. This study suggests that TLR2 is a pivotal epithelial barrier modulator, and provides novel insight in the molecular mechanisms by which LAB contribute to intestinal health.

    Originele taal-2English
    Pagina's (van-tot)1230-1234
    Aantal pagina's5
    TijdschriftFood & Function
    Volume11
    Nummer van het tijdschrift2
    DOI's
    StatusPublished - 11-feb-2020

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