Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex

I. Kusters; G. van den Bogaart; J.  de Wit; V. Krasnikov; B. Poolman; A. Driessen

doi:10.1007/978-1-60327-412-8_8

Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex

I. Kusters
, G. van den Bogaart
, J. de Wit
, V. Krasnikov
, B. Poolman
, A. Driessen

Onderzoeksoutput: Chapter › Academic › peer review

9 Citaten (Scopus)

1 Downloads (Pure)

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In bacteria, proteins are secreted across the cytoplasmic membrane by a protein complex termed translocase. The ability to study the activity of the translocase in vitro using purified proteins has been instrumental for our understanding of the mechanisms underlying this process. Here, we describe the protocols for the purification and reconstitution of the SecYEG complex in an active state into liposomes. In addition, fluorescence based in vitro assays are described that allow monitoring translocation activity discontinuously and in real time.

Originele taal-2	English
Titel	Protein Secretion
Subtitel	Methods and Protocols
Redacteuren	Anastassios Economou
Uitgeverij	Humana Press
Hoofdstuk	8
Pagina's	131-143
Aantal pagina's	13
Volume	619
ISBN van elektronische versie	978-1-60327-412-8
ISBN van geprinte versie	978-1-60327-167-7, 978-1-4939-5707-1
DOI's	https://doi.org/10.1007/978-1-60327-412-8_8
Status	Published - 25-jan.-2010

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abstract = "In bacteria, proteins are secreted across the cytoplasmic membrane by a protein complex termed translocase. The ability to study the activity of the translocase in vitro using purified proteins has been instrumental for our understanding of the mechanisms underlying this process. Here, we describe the protocols for the purification and reconstitution of the SecYEG complex in an active state into liposomes. In addition, fluorescence based in vitro assays are described that allow monitoring translocation activity discontinuously and in real time.",

author = "I. Kusters and Bogaart, \{G. van den\} and \{de Wit\}, J. and V. Krasnikov and B. Poolman and A. Driessen",

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T1 - Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex

AU - Kusters, I.

AU - Bogaart, G. van den

AU - de Wit, J.

AU - Krasnikov, V.

AU - Poolman, B.

AU - Driessen, A.

N1 - Relation: http://www.springerlink.com/ Rights: Springer

PY - 2010/1/25

Y1 - 2010/1/25

N2 - In bacteria, proteins are secreted across the cytoplasmic membrane by a protein complex termed translocase. The ability to study the activity of the translocase in vitro using purified proteins has been instrumental for our understanding of the mechanisms underlying this process. Here, we describe the protocols for the purification and reconstitution of the SecYEG complex in an active state into liposomes. In addition, fluorescence based in vitro assays are described that allow monitoring translocation activity discontinuously and in real time.

AB - In bacteria, proteins are secreted across the cytoplasmic membrane by a protein complex termed translocase. The ability to study the activity of the translocase in vitro using purified proteins has been instrumental for our understanding of the mechanisms underlying this process. Here, we describe the protocols for the purification and reconstitution of the SecYEG complex in an active state into liposomes. In addition, fluorescence based in vitro assays are described that allow monitoring translocation activity discontinuously and in real time.

U2 - 10.1007/978-1-60327-412-8_8

DO - 10.1007/978-1-60327-412-8_8

M3 - Chapter

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SN - 978-1-4939-5707-1

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PB - Humana Press

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Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex

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