Quantification of liver glucose metabolism by positron emission tomography: Validation study in pigs

Patricia Iozzo*, Mikko J. Jarvisalo, Jan Kiss, Ronald Borra, Gratian A. Naum, Antti Viljanen, Tapio Viljanen, Amalia Gastaldelli, Emma Buzzigoli, Letizia Guiducci, Elisabetta Barsotti, Timo Savunen, Juhani Knuuti, Merja Haaparanta-Solin, Ele Ferrannini, Pirjo Nuutila

*Corresponding author voor dit werk

    OnderzoeksoutputAcademicpeer review

    62 Citaten (Scopus)

    Samenvatting

    Background & Aims: The liver is inaccessible to organ balance measurements in humans. To validate [F-18]fluorodeoxyglucose ([F-18]FDG) positron emission tomography (PET) in the quantification of hepatic glucose uptake (HGU), we determined [F-18]FDG modeling parameters, lumped constant (LC), and input functions (single arterial versus dual). Methods: Anesthetized pigs were studied during fasting (n = 6), physiologic (n = 4), and supraphysiologic (n = 4) hyperinsulinemia. PET was performed with (CO)-O-15 (blood pool) and [F-18]FDG (glucose uptake). 6,6-Deuterated glucose ([H-2]G) was coinjected with [F-18]FDG and blood collected from the carotid artery and portal and hepatic veins to compute LC as ratio between tracers fractional extraction. HGU was estimated from PET images and ex vivo from high-performance liquid chromatography measurements of liver [F-18]FDG versus [F-18]FDG-6-phosphate and [F-18]glycogen. Endogenous glucose production was measured with [H-2]G and hepatic blood flow by flowmeters. Results: HGU was increased in hyperinsulinemia versus fasting (P <.05). Fractional extraction of [F-18]FDG and [H-2]G was similar (not significant), intercorrelated (r = 0.98, P <.0001), and equally higher during hyperinsulinemia than fasting (P 0.95, P <.0001), with a modest underestimation of HGU by the former. Conclusions: [F-18]FDG-PET-derived parameters provide accurate quantification of HGU and estimates of liver perfusion and glucose production. In the liver, LC of [F-18]FDG is nearly unitary. Using a single arterial input introduces only a small error in estimation of HGU.

    Originele taal-2English
    Pagina's (van-tot)531-542
    Aantal pagina's12
    TijdschriftGastroenterology
    Volume132
    Nummer van het tijdschrift2
    DOI's
    StatusPublished - feb.-2007

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