RalGEF2, a Pleckstrin Homology Domain Containing Guanine Nucleotide Exchange Factor for Ral

K M de Bruyn, J de Rooij, R M Wolthuis, H Rehmann, J Wesenbeek, R H Cool, A H Wittinghofer, J L Bos*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

35 Citaten (Scopus)
1 Downloads (Pure)


Ral is a ubiquitously expressed Ras-like small GTPase. Several guanine nucleotide exchange factors for Ral have been identified, including members of the RalGDS family, which exhibit a Ras binding domain and are regulated by binding to RasGTP. Here we describe a novel type of RalGEF, RalGEF2. This guanine nucleotide exchange factor has a characteristic Cdc25-like catalytic domain at the N terminus and a pleckstrin homology (PH) domain at the C terminus. RalGEF2 is able to activate Ral both in vivo and in vitro. Deletion of the PH domain results in an increased cytoplasmic localization of the protein and a corresponding reduction in activity in vivo, suggesting that the PH domain functions as a membrane anchor necessary for optimal activity in vivo.

Originele taal-2English
Pagina's (van-tot)29761-6
Aantal pagina's6
TijdschriftThe Journal of Biological Chemistry
Nummer van het tijdschrift38
StatusPublished - 22-sep-2000
Extern gepubliceerdJa

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