Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR

Ruud H Deurenberg; Cornelis Vink; Christel Driessen; Michèle Bes; Nancy London; Jerome Etienne; Ellen E Stobberingh

doi:10.1016/j.femsle.2004.09.031

Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR

Ruud H Deurenberg
, Cornelis Vink
, Christel Driessen
, Michèle Bes
, Nancy London
, Jerome Etienne
, Ellen E Stobberingh

Faculteit Medische Wetenschappen/UMCG

Onderzoeksoutput › Academic › peer review

38 Citaten (Scopus)

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To allow rapid identification of Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus strains, a real-time PCR assay for detection of PVL was developed. This assay is convenient, since it can be applied directly on bacterial suspensions and does not require previous DNA purification. Furthermore, the assay was found to be highly reproducible, robust and specific, since positive results were generated exclusively with PVL-positive S. aureus strains, and neither with PVL-negative strains nor staphylococci other than S. aureus.

Originele taal-2	English
Pagina's (van-tot)	225-228
Aantal pagina's	4
Tijdschrift	FEMS Microbiology Letters
Volume	240
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1016/j.femsle.2004.09.031
Status	Published - 15-nov.-2004

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@article{d7ef40580a64453593c707a9fc35cd6a,

title = "Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR",

abstract = "To allow rapid identification of Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus strains, a real-time PCR assay for detection of PVL was developed. This assay is convenient, since it can be applied directly on bacterial suspensions and does not require previous DNA purification. Furthermore, the assay was found to be highly reproducible, robust and specific, since positive results were generated exclusively with PVL-positive S. aureus strains, and neither with PVL-negative strains nor staphylococci other than S. aureus.",

keywords = "Bacterial Toxins, Exotoxins, Humans, Leukocidins, Polymerase Chain Reaction, Reproducibility of Results, Sensitivity and Specificity, Staphylococcal Infections, Staphylococcus aureus",

author = "Deurenberg, \{Ruud H\} and Cornelis Vink and Christel Driessen and Mich{\`e}le Bes and Nancy London and Jerome Etienne and Stobberingh, \{Ellen E\}",

year = "2004",

month = nov,

day = "15",

doi = "10.1016/j.femsle.2004.09.031",

language = "English",

volume = "240",

pages = "225--228",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "2",

}

TY - JOUR

T1 - Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR

AU - Deurenberg, Ruud H

AU - Vink, Cornelis

AU - Driessen, Christel

AU - Bes, Michèle

AU - London, Nancy

AU - Etienne, Jerome

AU - Stobberingh, Ellen E

PY - 2004/11/15

Y1 - 2004/11/15

N2 - To allow rapid identification of Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus strains, a real-time PCR assay for detection of PVL was developed. This assay is convenient, since it can be applied directly on bacterial suspensions and does not require previous DNA purification. Furthermore, the assay was found to be highly reproducible, robust and specific, since positive results were generated exclusively with PVL-positive S. aureus strains, and neither with PVL-negative strains nor staphylococci other than S. aureus.

AB - To allow rapid identification of Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus strains, a real-time PCR assay for detection of PVL was developed. This assay is convenient, since it can be applied directly on bacterial suspensions and does not require previous DNA purification. Furthermore, the assay was found to be highly reproducible, robust and specific, since positive results were generated exclusively with PVL-positive S. aureus strains, and neither with PVL-negative strains nor staphylococci other than S. aureus.

KW - Bacterial Toxins

KW - Exotoxins

KW - Humans

KW - Leukocidins

KW - Polymerase Chain Reaction

KW - Reproducibility of Results

KW - Sensitivity and Specificity

KW - Staphylococcal Infections

KW - Staphylococcus aureus

U2 - 10.1016/j.femsle.2004.09.031

DO - 10.1016/j.femsle.2004.09.031

M3 - Article

C2 - 15522511

SN - 0378-1097

VL - 240

SP - 225

EP - 228

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR

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