Rapid large scale fractionation of lymphoid cells by velocity sedimentation at unit gravity, using percoll as separation medium

D. Opstelten*, G. J. Deenen, L. Bos, A. Hermse, L. Muurman, P. Nieuwenhuis

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    8 Citaten (Scopus)

    Samenvatting

    Available methods for separation of cells by velocity sedimentation at unit gravity allow a maximum cell load of 2 × 108 lymphoid cells. Here we describe a method, based on the ‘tilting’ procedure of Bont et al. (1979), which allows separation of up to 4.5 × 109 lymphoid cells, while retaining full separation and rapidity. Percoll instead of Ficoll was used as gradient medium. The relationship between cell sedimentation velocity and cell volume was analyzed by two different methods, i.e., electronic cell sizing and diameter measurements of cytocentrifuged cells.
    Originele taal-2English
    Pagina's (van-tot)189-198
    Aantal pagina's10
    TijdschriftJournal of Immunological Methods
    Volume48
    Nummer van het tijdschrift2
    DOI's
    StatusPublished - 1982

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