Recombinant adeno-associated virus-based vectors provide short-term rather than long-term transduction of primitive hematopoietic stem cells

R van Os*, H Avraham, N Banu, PM Mauch, J Whater, YM Yang, B Du

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

8 Citaten (Scopus)
4 Downloads (Pure)

Samenvatting

Bone marrow stem cells collected from B6-Gpi-1(a) mice pretreated with 5-fluorouracil were incubated for 2 h at 37 degrees C in the presence of the recombinant adenovirus-associated virus-based vector (rAAV) SSV9, As measured in vitro immediately following transduction, SSV9 was found to be effective in transducing the primitive cobblestone-area-forming cell (CAFC)-35 subset (60% transduction efficiency). However, this did not predict long-term expression as the presence of the transgene could not be detected six months after transplantation of 1-2 x 10(6) transduced bone marrow stem cells into lethally irradiated recipients. CAFC analysis of bone marrow cells and Southern blot analysis of hone marrow and spleen cells were negative,and polymerase chain reaction analysis showed less than 0.1% transduction in bone marrow cells. Therefore, based on our study we conclude that rAAV transiently transduces hematopoietic stem cells but fails to integrate into the genome, leading to the loss of the reporter gene within the first six months after transplantation in vivo.

Originele taal-2English
Pagina's (van-tot)117-120
Aantal pagina's4
TijdschriftSTEM CELLS
Volume17
Nummer van het tijdschrift2
DOI's
StatusPublished - 1999
Extern gepubliceerdJa

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