Reducing virulence of the human pathogen Burkholderia by altering the substrate specificity of the quorum-quenching acylase PvdQ

Gudrun Koch, Pol Nadal-Jimenez, Carlos R. Reis, Remco Muntendam, Marcel Bokhove, Elena Melillo, Bauke W. Dijkstra, Robbert H. Cool, Wim J. Quax*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

44 Citaten (Scopus)


The use of enzymes to interfere with quorum sensing represents an attractive strategy to fight bacterial infections. We used PvdQ, an effective quorum-quenching enzyme from Pseudomonas aeruginosa, as a template to generate an acylase able to effectively hydrolyze C8-HSL, the major communication molecule produced by the Burkholderia species. We discovered that the combination of two single mutations leading to variant PvdQLα146W,Fβ24Y conferred high activity toward C8-HSL. Exogenous addition of PvdQLα146W,Fβ24Y dramatically decreased the amount of C8-HSL present in Burkholderia cenocepacia cultures and inhibited a quorum sensing-associated phenotype. The efficacy of this PvdQ variant to combat infections in vivo was further confirmed by its ability to rescue Galleria mellonella larvae upon infection, demonstrating its potential as an effective agent toward Burkholderia infections. Kinetic analysis of the enzymatic activities toward 3-oxo-C12-L-HSL and C8-L-HSL corroborated a substrate switch. This work demonstrates the effectiveness of quorum-quenching acylases as potential novel antimicrobial drugs. In addition, we demonstrate that their substrate range can be easily switched, thereby paving the way to selectively target only specific bacterial species inside a complex microbial community.
Originele taal-2English
Pagina's (van-tot)1568-1573
Aantal pagina's6
TijdschriftProceedings of the National Academy of Sciences of the United States of America
Nummer van het tijdschrift4
StatusPublished - 28-jan-2014

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