Reference intervals for Sysmex XN hematological parameters as assessed in the Dutch Lifelines cohort

Joost L. Van Pelt, Stefanie Klatte*, Talent Hwandih, Andrei Barcaru, Ineke J. Riphagen, Jo Linssen, Stephan J.L. Bakker

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

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Our aim was to derive reference intervals for all Sysmex XN hematology analyzer parameters. The rationale behind the study was the lack of reference intervals for the XN analyzer cell population data (CPD) and functional parameters. Fresh fasting blood samples from 18,484 participants in the Dutch Lifelines study were analyzed using two automated XN analyzers. Structured health questionnaire data were used to select a subgroup of 15,803 apparently healthy individuals for inclusion in the reference population. The Latent Abnormal Values Exclusion (LAVE) approach was used to reduce the influence of latent diseases in the reference population on the resulting reference intervals. We applied analysis of variance to judge the need for partitioning of the reference intervals by sex or age. We report reference intervals for 105 XN analyzer hematological parameters with and without applying LAVE. Sex-related partitioning was required for red blood cells, (RBC, RBC-O), hemoglobin (HGB, HGB-O), hematocrit (HCT), mean corpuscular hemoglobin concentration (MCHC), reticulocyte production index (RPI), and side scattered light intensity of the red blood cell population in the RET channel (RBC-Z). Partitioning for age was not warranted. Body mass index (BMI) and smoking had moderate influence on a minority of the parameters. We provide reference intervals for all Sysmex XN analyzer routine, CPD and functional parameters, using a direct approach in a large cohort in the Netherlands.

Originele taal-2English
Pagina's (van-tot)907–920
Aantal pagina's14
TijdschriftClinical chemistry and laboratory medicine
Nummer van het tijdschrift6
StatusPublished - 2-mei-2022

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