Regulation of Antibiotic Production by Bacterial Hormones

Nai-Hua Hsiao*, Marco Gottelt, Eriko Takano

*Corresponding author voor dit werk

OnderzoeksoutputAcademic

13 Citaten (Scopus)
401 Downloads (Pure)

Samenvatting

Antibiotic production is regulated by numerous signals, including the so-called bacterial hormones found in antibiotic producing organisms such as Streptomyces. These signals, the γ-butyrolactones, are produced in very small quantities, which has hindered their structural elucidation and made it difficult to assess whether they are being produced. In this chapter, we describe a rapid small-scale extraction method from either solid or liquid cultures in scales of one plate or 50 ml of medium. Also described is a bioassay to detect the γ-butyrolactones by determining either the production of pigmented antibiotic of Streptomyces coelicolor or kanamycin resistant growth on addition of the γ-butyrolactones. We also describe some insights into the identification of the γ-butyrolactone receptor and its targets and also the gel retardation conditions with three differently labeled probes.
Originele taal-2English
TitelCOMPLEX ENZYMES IN MICROBIAL NATURAL PRODUCT BIOSYNTHESIS, PART A: OVERVIEW ARTICLES AND PEPTIDES
RedacteurenDA Hopwood
Plaats van productieSAN DIEGO
UitgeverijAcademic Press
Pagina's143-157
Aantal pagina's15
ISBN van geprinte versie978-0-12-374588-0
DOI's
StatusPublished - 2009

Publicatie series

NaamMethods in Enzymology
UitgeverijELSEVIER ACADEMIC PRESS INC
Volume458
ISSN van geprinte versie0076-6879

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