Release of content through mechano-sensitive gates in pressurized liposomes

Martti Louhivuori*, Herre Risselada, Erik van der Giessen, Siewert J. Marrink

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

94 Citaten (Scopus)
314 Downloads (Pure)

Samenvatting

Mechano-sensitive channels are ubiquitous membrane proteins that activate in response to increasing tension in the lipid membrane. They facilitate a sudden, nonselective release of solutes and water that safeguards the integrity of the cell in hypo-or hyper-osmotic shock conditions. We have simulated the rapid release of content from a pressurized liposome through a particular mechano-sensitive protein channel, MscL, embedded in the liposomal membrane. We show that a single channel is able to relax the liposome, stressed to the point of bursting, in a matter of microseconds. We map the full activation-deactivation cycle of MscL in near-atomic detail and are able to quantify the rapid decrease in liposomal stress as a result of channel activation. This provides a computational tool that opens the way to contribute to the rational design of functional nano-containers.

Originele taal-2English
Pagina's (van-tot)19856-19860
Aantal pagina's5
TijdschriftProceedings of the National Academy of Sciences of the United States of America
Volume107
Nummer van het tijdschrift46
DOI's
StatusPublished - 16-nov-2010

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