Rieske Oxygenases and other Ferredoxin‐dependent Enzymes: Electron Transfer Principles and Catalytic Capabilities

Michael Ernst Runda, Niels A. W. De kok, Sandy Schmidt*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

3 Citaten (Scopus)
33 Downloads (Pure)


Enzymes that depend on sophisticated electron transfer via ferredoxins (Fds) exhibit outstanding catalytic capabilities, but despite decades of research, many of them are still not well understood nor exploited for synthetic applications. This review aims to provide a general overview of the most important Fd-dependent enzymes and the electron transfer processes involved. While several examples are discussed, we focus in particular on the family of Rieske non-heme iron-dependent oxygenases (ROs). In addition to illustrating their electron transfer principles and catalytic potential, the current state of knowledge on structure-function relationships and the mode of interaction between the redox partner proteins is reviewed. Moreover, we highlight several key catalyzed transformations, but also take a deeper dive into their engineerability for biocatalytic applications. The overall findings from these case studies highlight the catalytic capabilities of these biocatalysts and may stimulate future interest in developing additional Fd-dependent enzyme classes for synthetic applications.
Originele taal-2English
Aantal pagina's23
Nummer van het tijdschrift15
Vroegere onlinedatum25-mrt.-2023
StatusPublished - 1-aug.-2023

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