RNA Framework for Assaying the Structure of RNAs by High-Throughput Sequencing

Tycho Marinus, Danny Incarnato*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

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RNA structure is a key player in regulating a plethora of biological processes. A large part of the functions carried out by RNA is mediated by its structure. To this end, in the last decade big effort has been put in the development of new RNA probing methods based on Next-Generation Sequencing (NGS), aimed at the rapid transcriptome-scale interrogation of RNA structures. In this chapter we describe RNA Framework, the to date most comprehensive toolkit for the analysis of NGS-based RNA structure probing experiments. By using two published datasets, we here illustrate how to use the different components of the RNA Framework and how to choose the analysis parameters according to the experimental setup.

Originele taal-2English
TitelRNA Bioinformatics
RedacteurenE. Picardi
Plaats van productieNew York
UitgeverijHumana, New York, NY
Hoofdstuk5
Pagina's63-76
Aantal pagina's14
ISBN van elektronische versie978-1-0716-1307-8
ISBN van geprinte versie978-1-0716-1306-1
DOI's
StatusPublished - 2021

Publicatie series

NaamMethods in Molecular Biology
UitgeverijSPRINGER
Volume2284
ISSN van geprinte versie1064-3745

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