TY - JOUR
T1 - Rolling-circle plasmids from Bacillus subtilis
T2 - complete nucleotide sequences and analyses of genes of pTA1015, pTA1040, pTA1050 and pTA1060, and comparisons with related plasmids from Gram-positive bacteria
AU - Meijer, W.J.
AU - Schuurs-Wisman, Bea
AU - Terpstra, P
AU - Thorsted, P.
AU - Thomas, C.M.
AU - Holsappel, S
AU - Venema, G
AU - Bron, S
N1 - Relation: http://www.rug.nl/gbb/
date_submitted:2009
Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute
PY - 1998/2
Y1 - 1998/2
N2 - Most small plasmids of Gram-positive bacteria use the rolling-circle mechanism of replication and several of these have been studied in considerable detail at the DNA level and for the function of their genes. Although most of the common laboratory Bacillus subtilis 168 strains do not contain plasmids, several industrial strains and natural soil isolates do contain rolling-circle replicating (RCR) plasmids. So far, knowledge about these plasmids was mainly limited to: (i) a classification into seven groups, based on size and restriction patterns; and (ii) DNA sequences of the replication region of a limited number of them. To increase the knowledge, also with respect to other functions specified by these plasmids, we have determined the complete DNA sequence of four plasmids, representing different groups, and performed computer-assisted and experimental analyses on the possible function of their genes. The plasmids analyzed are pTA1015 (5.8 kbp), pTA1040 (7.8 kbp), pTA1050 (8.4 kbp), and pTA1060 (8.7 kbp). These plasmids have a structural organization similar to most other known RCR plasmids. They contain highly related replication functions, both for leading and lagging strand synthesis. pTA1015 and pTA1060 contain a mobilization gene enabling their conjugative transfer. Strikingly, in addition to the conserved replication modules, these plasmids contain unique module(s) with genes which are not present on known RCR plasmids of other Gram-positive bacteria. Examples are genes encoding a type I signal peptidase and genes encoding proteins belonging to the family of response regulator aspartate phosphatases. The latter are likely to be involved in the regulation of post-exponential phase processes. The presence of these modules on plasmids may reflect an adaptation to the special conditions to which the host cells were exposed. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.
AB - Most small plasmids of Gram-positive bacteria use the rolling-circle mechanism of replication and several of these have been studied in considerable detail at the DNA level and for the function of their genes. Although most of the common laboratory Bacillus subtilis 168 strains do not contain plasmids, several industrial strains and natural soil isolates do contain rolling-circle replicating (RCR) plasmids. So far, knowledge about these plasmids was mainly limited to: (i) a classification into seven groups, based on size and restriction patterns; and (ii) DNA sequences of the replication region of a limited number of them. To increase the knowledge, also with respect to other functions specified by these plasmids, we have determined the complete DNA sequence of four plasmids, representing different groups, and performed computer-assisted and experimental analyses on the possible function of their genes. The plasmids analyzed are pTA1015 (5.8 kbp), pTA1040 (7.8 kbp), pTA1050 (8.4 kbp), and pTA1060 (8.7 kbp). These plasmids have a structural organization similar to most other known RCR plasmids. They contain highly related replication functions, both for leading and lagging strand synthesis. pTA1015 and pTA1060 contain a mobilization gene enabling their conjugative transfer. Strikingly, in addition to the conserved replication modules, these plasmids contain unique module(s) with genes which are not present on known RCR plasmids of other Gram-positive bacteria. Examples are genes encoding a type I signal peptidase and genes encoding proteins belonging to the family of response regulator aspartate phosphatases. The latter are likely to be involved in the regulation of post-exponential phase processes. The presence of these modules on plasmids may reflect an adaptation to the special conditions to which the host cells were exposed. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.
KW - Bacillus subtilis
KW - rolling-circle plasmid
KW - mobilization
KW - response aspartate phosphatase
KW - signal peptidase
KW - replication module
KW - SIGNAL PEPTIDASE-I
KW - STREPTOGRAMIN-B RESISTANCE
KW - PROTEIN ASPARTATE PHOSPHATASES
KW - GLUTAMYL-TRANSPEPTIDASE GENE
KW - SINGLE-STRANDED-DNA
KW - STAPHYLOCOCCUS-AUREUS
KW - STREPTOCOCCAL PLASMID
KW - ESCHERICHIA-COLI
KW - NATTO PLASMID
KW - REPLICATION ORIGIN
U2 - 10.1111/j.1574-6976.1998.tb00357.x
DO - 10.1111/j.1574-6976.1998.tb00357.x
M3 - Article
SN - 0168-6445
VL - 21
SP - 337
EP - 368
JO - FEMS Microbiology Reviews
JF - FEMS Microbiology Reviews
IS - 4
ER -