RTEL1 contributes to DNA replication and repair and telomere maintenance

Evert-Jan Uringa, Kathleen Lisaingo, Hilda A. Pickett, Julie Brind'Amour, Jan-Hendrik Rohde, Alex Zelensky, Jeroen Essers, Peter M. Lansdorp*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

92 Citaten (Scopus)
295 Downloads (Pure)


Telomere maintenance and DNA repair are important processes that protect the genome against instability. mRtel1, an essential helicase, is a dominant factor setting telomere length in mice. In addition, mRtel1 is involved in DNA double-strand break repair. The role of mRtel1 in telomere maintenance and genome stability is poorly understood. Therefore we used mRtel1-deficient mouse embryonic stem cells to examine the function of mRtel1 in replication, DNA repair, recombination, and telomere maintenance. mRtel1-deficient mouse embryonic stem cells showed sensitivity to a range of DNA-damaging agents, highlighting its role in replication and genome maintenance. Deletion of mRtel1 increased the frequency of sister chromatid exchange events and suppressed gene replacement, demonstrating the involvement of the protein in homologous recombination. mRtel1 localized transiently at telomeres and is needed for efficient telomere replication. Of interest, in the absence of mRtel1, telomeres in embryonic stem cells appeared relatively stable in length, suggesting that mRtel1 is required to allow extension by telomerase. We propose that mRtel1 is a key protein for DNA replication, recombination, and repair and efficient elongation of telomeres by telomerase.

Originele taal-2English
Pagina's (van-tot)2782-2792
Aantal pagina's11
TijdschriftMolecular Biology of the Cell
Nummer van het tijdschrift14
StatusPublished - 15-jul.-2012

Citeer dit