Samenvatting
In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes. Screening of the GenBank database resulted in the identification of a corresponding gene or EST for 490 of them. For 211 of the tags no homology could be detected. A major problem of the serial analysis of gene expression (SAGE) approach is how to further analyse the unknown tags. We have developed an RT-PCR-based method, rapid analysis of unknown SAGE tags (RAST-PCR), to analyse the expression of the corresponding genes. This approach can be used as a screening method to investigate whether or not the gene is differentially expressed between several cell types of interest.
| Originele taal-2 | English |
|---|---|
| Pagina's (van-tot) | e17 |
| Tijdschrift | Nucleic Acids Research |
| Volume | 27 |
| Nummer van het tijdschrift | 17 |
| Status | Published - 1-sep.-1999 |