SPATIAL AND TEMPORAL ACCUMULATION OF MESSENGER-RNAS ENCODING 2 COMMON LIGNIN PEROXIDASES IN PHANEROCHAETE-CHRYSOSPORIUM

SM MOUKHA*, HAB WOSTEN, EJ MYLIUS, M ASTHER, JGH WESSELS

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    21 Citaten (Scopus)

    Samenvatting

    Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both transcripts spread to more-peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA.

    Originele taal-2English
    Pagina's (van-tot)3672-3678
    Aantal pagina's7
    TijdschriftJournal of Bacteriology
    Volume175
    Nummer van het tijdschrift11
    StatusPublished - jun-1993

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