SPEC® disc solid-phase extraction for rapid broad-spectrum drug screening in urine

R.A de Zeeuw*, J Wijsbeek, J.P. Franke

*Bijbehorende auteur voor dit werk

Onderzoeksoutput: ArticleAcademicpeer review

16 Citaten (Scopus)


Broad-spectrum drug screening requires that all relevant substances be isolated, detected, and identified, regardless of their structure and/or polarity. To this end, systematic solid-phase extraction (SPE) approaches for drug isolation from biological fluids are required. Because speed and cost effectiveness are key issues in analytical toxicology, we have evaluated a disc-format extraction device for this purpose and compared the latter with an existing packed-bed column-format method. The discs were SPEC.PLUS.C18AR/MP3 cartridges with 10-mL solvent reservoirs, providing hydrophobic and cation exchange interactions. Blank human urine was spiked at 2 µg/mL with a selection of acidic, neutral, and basic drugs representing a variety of relevant drug classes. Urine specimens (2 mL) were diluted with 2 mL 0.1M phosphate buffer (pH 5.0) and then applied to the preconditioned disc. Washing was done with 1 mL water. Acidic and neutral drugs were eluted with 1 mL ethyl acetate/acetone (1:1), and basic drugs were eluted with 1 mL ammoniated ethyl acetate. The eluates were collected separately, evaporated down to about 0.1 mL, and analyzed by gas chromatography-flame-ionization detection to check cleanliness, recoveries, and reproducibilities. The discs showed good extraction properties for all drugs and were easy to handle. Recoveries were 75–100% with coefficients of variation of around 5%. The resulting eluates showed only a few matrix interferences. As compared to our standard SPE method with packed-bed columns, the disc procedure allowed reductions in elution volumes and total processing time of approximately 60–65%.
Originele taal-2English
Pagina's (van-tot)97-101
Aantal pagina's5
TijdschriftJournal of Analytical Toxicology
Nummer van het tijdschrift2
StatusPublished - mrt.-2000
EvenementSOFT/TIAFT 98 Meeting -
Duur: 5-okt.-19989-okt.-1998

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