Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells

Anika Nagelkerke; Paul N Span

doi:10.1007/978-3-319-26666-4_1

Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells

Anika Nagelkerke, Paul N Span

Onderzoeksoutput › Academic › peer review

59 Citaten (Scopus)

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Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we provide a protocol to perform immunostaining for phospho-H2AX on cells, cryosections and formalin-fixed, paraffin-embedded tissues. Crucial steps in the protocol and troubleshooting suggestions are indicated. We also provide suggestions on how to combine staining against γ-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels.

Originele taal-2	English
Pagina's (van-tot)	1-10
Aantal pagina's	10
Tijdschrift	Advances in experimental medicine and biology
Volume	899
DOI's	https://doi.org/10.1007/978-3-319-26666-4_1
Status	Published - 22-jun.-2016
Extern gepubliceerd	Ja

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title = "Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells",

abstract = "Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we provide a protocol to perform immunostaining for phospho-H2AX on cells, cryosections and formalin-fixed, paraffin-embedded tissues. Crucial steps in the protocol and troubleshooting suggestions are indicated. We also provide suggestions on how to combine staining against γ-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels. ",

keywords = "Biomarkers/metabolism, Cryoultramicrotomy, DNA Damage, Genomic Instability, Histones/metabolism, Humans, Neoplasms/genetics, Paraffin Embedding, Phosphorylation, Staining and Labeling/methods, Tissue Fixation",

author = "Anika Nagelkerke and Span, {Paul N}",

year = "2016",

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doi = "10.1007/978-3-319-26666-4_1",

language = "English",

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T1 - Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells

AU - Nagelkerke, Anika

AU - Span, Paul N

PY - 2016/6/22

Y1 - 2016/6/22

N2 - Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we provide a protocol to perform immunostaining for phospho-H2AX on cells, cryosections and formalin-fixed, paraffin-embedded tissues. Crucial steps in the protocol and troubleshooting suggestions are indicated. We also provide suggestions on how to combine staining against γ-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels.

AB - Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we provide a protocol to perform immunostaining for phospho-H2AX on cells, cryosections and formalin-fixed, paraffin-embedded tissues. Crucial steps in the protocol and troubleshooting suggestions are indicated. We also provide suggestions on how to combine staining against γ-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels.

KW - Biomarkers/metabolism

KW - Cryoultramicrotomy

KW - DNA Damage

KW - Genomic Instability

KW - Histones/metabolism

KW - Humans

KW - Neoplasms/genetics

KW - Paraffin Embedding

KW - Phosphorylation

KW - Staining and Labeling/methods

KW - Tissue Fixation

U2 - 10.1007/978-3-319-26666-4_1

DO - 10.1007/978-3-319-26666-4_1

M3 - Article

C2 - 27325258

SN - 0065-2598

VL - 899

SP - 1

EP - 10

JO - Advances in experimental medicine and biology

JF - Advances in experimental medicine and biology

ER -

Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells

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