Staining sections of water‐miscible resins 2. Effects of staining‐reagent lipophilicity on the staining of glycol‐methacrylate‐embedded tissues

R.W. Horobin*, P.O. Gerrits, D.J. Wright

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

    8 Citaten (Scopus)

    Samenvatting

    Glycol methacrylate (GMA) sections of animal tissues were stained with a group of twenty-seven reagents of very varied chemical characteristics. The artefactual background staining of the resin was found to be dependent on the hydrophilic/lipophilic character of the staining reagent, as estimated from the logarithm of its octanol-water partition coefficient (log P). Intense background staining occurred with lipophilic stains, whose log P > 2. In keeping with this, use of GMA semi-permeable membranes for enzyme histochemistry failed to give staining when using a lipophilic substrate, probably because the substrate was trapped in the membrane. An analysis of other routine histochemical stains-in terms of the probable occurrence of high resin background staining and low tissue sensitivity-is made. A numerical guide is provided to help avoid artefacts resulting from hydrophobic and size effects. Note: small, hydrophilic reagents (log P <0; molecular weight <550 Da) are least likely to show either type of artefact. Conversely, reagents which are lipophilic, or/and of intermediate size (log P > 2; 550 <ionic weight <1000 Da), give strong background staining.

    Originele taal-2English
    Pagina's (van-tot)199-205
    Aantal pagina's7
    TijdschriftJournal of microscopy-Oxford
    Volume166
    DOI's
    StatusPublished - mei-1992

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