Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes

Gang Xiang, Hans Leemhuis, Marc Jos Elise Cornelis van Der Maarel*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

4 Citaten (Scopus)
8 Downloads (Pure)


Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an alpha-1,6 glycosidic bond between alpha-1,4 linked glucooliogosaccharides. As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far. This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs. A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two beta-strands.

Originele taal-2English
Pagina's (van-tot)155-163
Aantal pagina's9
TijdschriftProteins-Structure Function and Bioinformatics
Nummer van het tijdschrift1
Vroegere onlinedatum9-aug-2021
StatusPublished - jan-2022

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