Structure of Pex5p and Pex5-20 complexes in the yeast Hansenula polymorpha. Pex20p causes a conformational change upon binding to Pex5p tetramers involved in peroxisomal protein transport.

Kasia Moscicka, Sandra H. Klompmaker, Dongyuan Wang, Ida J. van der Klei, Egbert J. Boekema


187 Downloads (Pure)


Peroxisomal matrix proteins are synthesized on free polyribosomes and directed to the organelle by specific peroxisomal targeting signals (PTSs).
The PEX5 gene encodes the PTS1 receptor, Pex5p, which interacts with the PTS1 signal via a series of tetratricopeptide repeats (TPRs) within its C terminus. A crystal structure has been determined of a 41 kDa fragment of human Pex5p that includes six TPR motifs in complex with a small peptide containing a PTS1 sequence [1,2] or the sterol carrier protein [3]. This structure reveals the molecular basis for PTS1 recognition which is mostly formed by two clusters of three TPRs almost completely surrounding the PTS1-peptide.
However, whether or not Pex5p functions as an oligomer, is still a matter of debate. Gel filtration chromatography and electron microscopy studies indicated that human Pex5p (HsPex5p) is a homotetramer [4]. Fluorescence spectroscopy studies on Pex5p of the yeast Hansenula polymorpha (HpPex5p) indicated that HpPex5p also forms oligomers [5].
In this study, the projection structures of HpPex5p and HpPex5p-HpPex20p complexes were investigated by single particle electron microscopy. The analysis shows that HpPex5p is a tetramer and that HpPex20p is able to induce a major conformational change leading to a rather open space in the centre of the HpPex5p tetramer. In a successive set of experiments we show that HpPex5p-HpPex20p complexes are able to bind folded copies of tetrameric catalase at the periphery. Since catalase is one of the major peroxisomal proteins this indicates that such HpPex5p-HpPex20p-catalase complexes are functional as receptor complex.
Originele taal-2English
Aantal pagina's2
StatusPublished - 2008
EvenementEMC 2008 -
Duur: 1-jan-2008 → …


ConferenceEMC 2008
Periode01/01/2008 → …

Citeer dit