Systematic In Vivo Characterization of Fluorescent Protein Maturation in Budding Yeast

Paolo Guerra, Luc-Alban Vuillemenot, Brady Rae, Valeriia Ladyhina, Andreas Milias-Argeitis*

*Corresponding author voor dit werk

OnderzoeksoutputAcademicpeer review

6 Citaten (Scopus)
87 Downloads (Pure)


Fluorescent protein (FP) maturation can limit the accuracy with which dynamic intracellular processes are captured and reduce the in vivo brightness of a given FP in fast-dividing cells. The knowledge of maturation timescales can therefore help users determine the appropriate FP for each application. However, in vivo maturation rates can greatly deviate from in vitro estimates that are mostly available. In this work, we present the first systematic study of in vivo maturation for 12 FPs in budding yeast. To overcome the technical limitations of translation inhibitors commonly used to study FP maturation, we implemented a new approach based on the optogenetic stimulations of FP expression in cells grown under constant nutrient conditions. Combining the rapid and orthogonal induction of FP transcription with a mathematical model of expression and maturation allowed us to accurately estimate maturation rates from microscopy data in a minimally invasive manner. Besides providing a useful resource for the budding yeast community, we present a new joint experimental and computational approach for characterizing FP maturation, which is applicable to a wide range of organisms.

Originele taal-2English
Pagina's (van-tot)1129-1141
Aantal pagina's13
TijdschriftACS Synthetic Biology
Nummer van het tijdschrift3
Vroegere onlinedatum18-feb.-2022
StatusPublished - apr.-2022


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