Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Jonas Lohse, Alexandra Schindl, Natasha Danda, Chris P Williams, Karl Kramer, Bernhard Kuster, Martin D Witte*, Guillaume Médard*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

3 Citaten (Scopus)
27 Downloads (Pure)

Samenvatting

A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

Originele taal-2English
Pagina's (van-tot)11929-11932
Aantal pagina's4
TijdschriftChem. Commun.
Volume53
Nummer van het tijdschrift87
DOI's
StatusPublished - 11-nov-2017

Citeer dit