The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF complex for proliferation arrest

Christine Müller, Cornelis F Calkhoven, Xiaojing Sha, Achim Leutz

OnderzoeksoutputAcademicpeer review

74 Citaten (Scopus)
24 Downloads (Pure)

Samenvatting

The transcription factor CCAAT enhancer-binding protein alpha (C/EBPalpha) is a tumor suppressor in myeloid cells and inhibits proliferation in all cell types examined. C/EBPalpha interacts with the SWI/SNF chromatin-remodeling complex during the regulation of differentiation-specific genes. Here we show that C/EBPalpha fails to suppress proliferation in SWI/SNF defective cell lines after knock-down of SWI/SNF core components or after deletion of the SWI/SNF interaction domain in C/EBPalpha, respectively. Reconstitution of SWI/SNF function restores C/EBPalpha-dependent proliferation arrest. Our results show that the anti-proliferation activity of C/EBPalpha critically depends on components of the SWI/SNF core complex and suggest that the functional interaction between SWI/SNF and C/EBPalpha is a prerequisite for proliferation arrest.

Originele taal-2English
Pagina's (van-tot)7353-8
Aantal pagina's6
TijdschriftThe Journal of Biological Chemistry
Volume279
Nummer van het tijdschrift8
DOI's
StatusPublished - 20-feb.-2004
Extern gepubliceerdJa

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