The coronavirus nucleocapsid protein is dynamically associated with the replication-transcription complexes

Monique H Verheije, Marne C Hagemeijer, Mustafa Ulasli, Fulvio Reggiori, Peter J M Rottier, Paul S Masters, Cornelis A M de Haan

OnderzoeksoutputAcademicpeer review

73 Citaten (Scopus)
54 Downloads (Pure)


The coronavirus nucleocapsid (N) protein is a virion structural protein. It also functions, however, in an unknown way in viral replication and localizes to the viral replication-transcription complexes (RTCs). Here we investigated, using recombinant murine coronaviruses expressing green fluorescent protein (GFP)-tagged versions of the N protein, the dynamics of its interactions with the RTCs and the domain(s) involved. Using fluorescent recovery after photobleaching, we showed that the N protein, unlike the nonstructural protein 2, is dynamically associated with the RTCs. Recruitment of the N protein to the RTCs requires the C-terminal N2b domain, which interacts with other N proteins in an RNA-independent manner.

Originele taal-2English
Pagina's (van-tot)11575-11579
Aantal pagina's5
TijdschriftJournal of Virology
Nummer van het tijdschrift21
StatusPublished - nov.-2010
Extern gepubliceerdJa

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