The effect of aliphatic alcohols on fluid bilayers in unilamellar DOPC vesicles - A small-angle neutron scattering and molecular dynamics study

M. Klacsova*, M. Bulacu, N. Kucerka, D. Uhrikova, J. Teixeira, S. J. Marrink, P. Balgavy

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

27 Citaten (Scopus)
358 Downloads (Pure)


Small-angle neutron scattering and coarse-grained molecular dynamics simulations have been used to determine the structural parameters (bilayer thickness D, polar region thickness D(H), interfacial lateral area of the unit cell A(UC) and alcohol partial interfacial area A(CnOH)) of fluid dioleoylphosphatidylcholine:dioleoyl phosphatidylserine (PCPS, DOPC:DOPS = 24.7 mol:mol) bilayers in extruded unilamellar vesicles with incorporated aliphatic alcohols (CnOH, n = 8-18 is the even number of carbons in alkyl chain). External (2)H(2)O/H(2)O contrast variation experiments revealed that D(H) decreases as a function of alkyl chain length and CnOH:PCPS molar ratio. Using measurements at single 100% (2)H(2)O contrast we found that (i) D decreases with CnOH:PCPS molar ratio and increases with CnOH chain length (at 0.4 molar ratio); (ii) A(UC) significantly increases already in the presence of shortest CnOH studied (at 0.4 molar ratio), further increase is observed with longer CnOHs and at higher molar ratios: (iii) A(CnOH) of alcohol molecules in PCPS bilayer increases linearly with the alkyl chain length, A(CnOH) obtained for CnOHs with n

Originele taal-2English
Pagina's (van-tot)2136-2146
Aantal pagina's11
TijdschriftBiochimica et Biophysica Acta-Biomembranes
Nummer van het tijdschrift9
StatusPublished - sep-2011

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