The interaction of human endothelial cells with chemical gradient surfaces during exposure to flow

TG Ruardy, HE Moorlag, JM Schakenraad, J Van der Meer, HC Van der Mei, HJ Busscher*

*Bijbehorende auteur voor dit werk

    Onderzoeksoutput: Conference contributionAcademicpeer review


    In this study, the position bound shape, spreading, detachment and migration of adhering HUVEC endothelial cells on dichlorodimethylsilane (DDS) chemical gradient surfaces was investigated during exposure to flow in a parallel plate flow chamber in the presence of` serum proteins. Gradient surfaces were prepared by the diffusion method and characterized by the Wilhelmy plate technique for their wettability. Ln order to measure the possible influence of wettability steepness on the position bound cellular response. shallow and steep wettability gradients were prepared by applying two different diffusion times and DDS-concentrations. Quantitative analyses of the cellular response on the gradient surfaces showed that the position bound spread area and,shape Factor of adhering cells increased when going from the hydrophobic to the hydrophilic end, concurrent with the steepness of the gradient. After exposure to Row, cells detached immediately from the hydrophobic side and maintained their positions more easily on the hydrophilic side. Furthermore, it was shown that the pc:,position bound detachment and migration of cells depended on the steepness of` the wettability gradient and the direction of the flow. When the Row was to the hydrophilic end, migration velocity did not vary along the length of the gradients, and was lower on the steep (approximately 2 mu m/min) than on the shallow (approximately ly 3 mu m/min) gradients. However, when flow was to the hydropbobic end, migration velocities appeared position bound and were higher on the hydrophobic ends of both gradients.

    Originele taal-2English
    TitelInvited papers - First International Congress on Adhesion Science and Technology
    RedacteurenWJV Olij, HR Anderson
    Plaats van productieLEIDEN
    Aantal pagina's4
    ISBN van geprinte versie90-6764-291-6
    StatusPublished - 1998
    Evenement1st International Congress on Adhesion Science and Technology in Honor of Dr Kash Mittal on his 50th Birthday - , Netherlands
    Duur: 16-okt-199520-okt-1995


    Other1st International Congress on Adhesion Science and Technology in Honor of Dr Kash Mittal on his 50th Birthday

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