The ntp operon encoding the Na+V-ATPase of the thermophile Caloramator fervidus

Trees Ubbink-Kok, Jeroen Nijland, Dirk-Jan Slotboom, Juke S. Lolkema*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

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Samenvatting

The V-type ATPase of the thermophile Caloramator fervidus is an ATP-driven Na+ pump. The nucleotide sequence of the ntpFIKECGABD operon containing the structural genes coding for the nine subunits of the enzyme complex was determined. The identity of the proteins in two pairs of subunits (D, E and F, G) that have very similar mobilities on SDS-PAGE of the purified complex (24.3 and 22.7 kDa, and 12.3 and 11.6 kDa) was established by tryptic digestion of the protein bands followed by mass spectrometric analysis of the peptides.

Originele taal-2English
Pagina's (van-tot)513-517
Aantal pagina's5
TijdschriftArchives of Microbiology
Volume186
Nummer van het tijdschrift6
DOI's
StatusPublished - dec-2006

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