The quantitative and condition-dependent Escherichia coli proteome

Alexander Schmidt*, Karl Kochanowski, Silke Vedelaar, Erik Ahrné, Benjamin Volkmer, Luciano Callipo, Kèvin Knoops, Manuel Bauer, Ruedi Aebersold, Matthias Heinemann

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

396 Citaten (Scopus)
31 Downloads (Pure)

Samenvatting

Measuring precise concentrations of proteins can provide insights into biological processes. Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art quantitative mass spectrometry techniques to generate a comprehensive, condition-dependent protein-abundance map for Escherichia coli. We measure cellular protein concentrations for 55% of predicted E. coli genes (>2,300 proteins) under 22 different experimental conditions and identify methylation and N-terminal protein acetylations previously not known to be prevalent in bacteria. We uncover system-wide proteome allocation, expression regulation and post-translational adaptations. These data provide a valuable resource for the systems biology and broader E. coli research communities.

Originele taal-2English
Pagina's (van-tot)104-110
Aantal pagina's7
TijdschriftNature Biotechnology
Volume34
Nummer van het tijdschrift1
Vroegere onlinedatum7-dec-2015
DOI's
StatusPublished - jan-2016

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