Measuring precise concentrations of proteins can provide insights into biological processes. Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art quantitative mass spectrometry techniques to generate a comprehensive, condition-dependent protein-abundance map for Escherichia coli. We measure cellular protein concentrations for 55% of predicted E. coli genes (>2,300 proteins) under 22 different experimental conditions and identify methylation and N-terminal protein acetylations previously not known to be prevalent in bacteria. We uncover system-wide proteome allocation, expression regulation and post-translational adaptations. These data provide a valuable resource for the systems biology and broader E. coli research communities.
Schmidt, A. (Creator), Kochanowski, K. (Creator), Bonsing-Vedelaar, S. (Creator), Ahrne, E. (Creator), Volkmer, B. (Creator), Callipo, L. (Creator), Knoops, K. (Creator), Bauer, M. (Creator), Aebersold, R. (Creator) & Heinemann, M. (Creator), European Bioinformatics Institute (EMBL-EBI), 8-dec-2015