TY - JOUR
T1 - The role of gut microbiota in health and disease
T2 - In vitro modeling of host-microbe interactions at the aerobe-anaerobe interphase of the human gut
AU - von Martels, Julius Z. H.
AU - Sadabad, Mehdi Sadaghian
AU - Bourgonje, Arno R.
AU - Blokzijl, Tjasso
AU - Dijkstra, Gerard
AU - Faber, Klaas Nico
AU - Harmsen, Hermie J. M.
N1 - Copyright © 2017. Published by Elsevier Ltd.
PY - 2017/4
Y1 - 2017/4
N2 - The microbiota of the gut has many crucial functions in human health. Dysbiosis of the microbiota has been correlated to a large and still increasing number of diseases. Recent studies have mostly focused on analyzing the associations between disease and an aberrant microbiota composition. Functional studies using (in vitro) gut models are required to investigate the precise interactions that occur between specific bacteria (or bacterial mixtures) and gut epithelial cells. As most gut bacteria are obligate or facultative anaerobes, studying their effect on oxygen-requiring human gut epithelial cells is technically challenging. Still, several (anaerobic) bacterial-epithelial co-culture systems have recently been developed that mimic host-microbe interactions occurring in the human gut, including 1) the Transwell "apical anaerobic model of the intestinal epithelial barrier", 2) the Host-Microbiota Interaction (HMI) module, 3) the "Human oxygen-Bacteria anaerobic" (HoxBan) system, 4) the human gut-on-a-chip and 5) the HuMiX model. This review discusses the role of gut microbiota in health and disease and gives an overview of the characteristics and applications of these novel host-microbe co-culture systems. (C) 2017 Elsevier Ltd. All rights reserved.
AB - The microbiota of the gut has many crucial functions in human health. Dysbiosis of the microbiota has been correlated to a large and still increasing number of diseases. Recent studies have mostly focused on analyzing the associations between disease and an aberrant microbiota composition. Functional studies using (in vitro) gut models are required to investigate the precise interactions that occur between specific bacteria (or bacterial mixtures) and gut epithelial cells. As most gut bacteria are obligate or facultative anaerobes, studying their effect on oxygen-requiring human gut epithelial cells is technically challenging. Still, several (anaerobic) bacterial-epithelial co-culture systems have recently been developed that mimic host-microbe interactions occurring in the human gut, including 1) the Transwell "apical anaerobic model of the intestinal epithelial barrier", 2) the Host-Microbiota Interaction (HMI) module, 3) the "Human oxygen-Bacteria anaerobic" (HoxBan) system, 4) the human gut-on-a-chip and 5) the HuMiX model. This review discusses the role of gut microbiota in health and disease and gives an overview of the characteristics and applications of these novel host-microbe co-culture systems. (C) 2017 Elsevier Ltd. All rights reserved.
KW - Anaerobic gut bacteria
KW - Human intestinal epithelium
KW - Co-culture system
KW - Host-microbe interactions
KW - Transwell co-culture
KW - Host-Microbiota Interaction (HMI) module
KW - Human oxygen-Bacteria anaerobic
KW - (HoxBan) system
KW - The human gut-on-a-chip
KW - HuMiX model
KW - INFLAMMATORY-BOWEL-DISEASE
KW - INTESTINAL EPITHELIAL BARRIER
KW - HUMAN GASTROINTESTINAL-TRACT
KW - CONTINUOUS-CULTURE SYSTEM
KW - CROHNS-DISEASE
KW - FAECALIBACTERIUM-PRAUSNITZII
KW - COLONIC FERMENTATION
KW - CELIAC-DISEASE
KW - NONPATHOGENIC BACTERIA
KW - MAINTENANCE TREATMENT
U2 - 10.1016/j.anaerobe.2017.01.001
DO - 10.1016/j.anaerobe.2017.01.001
M3 - Review article
C2 - 28062270
SN - 1075-9964
VL - 44
SP - 3
EP - 12
JO - Anaerobe
JF - Anaerobe
ER -