The role of Hansenula polymorpha MIG1 homologues in catabolite repression and pexophagy

Olena G. Stasyk, Tim Van Zutphen, Huyn Ah Kang, Oleh V. Stasyk, Marten Veenhuis, Andriy A. Sibirny*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

22 Citaten (Scopus)
257 Downloads (Pure)

Samenvatting

In the methanol-utilizing yeast Hansenula polymorpha, glucose and ethanol trigger the repression of peroxisomal enzymes at the transcriptional level, and rapid and selective degradation of methanol-induced peroxisomes by means of a process termed pexophagy. In this report we demonstrate that deficiency in the putative H. polymorpha homologues of transcriptional repressors Mig1 (HpMig1 and HpMig2), as well as HpTup1, partially and differentially affects the repression of peroxisomal alcohol oxidase by sugars and ethanol. As reported earlier, deficiency in HpTup1 leads to impairment of glucose- or ethanol-induced macropexophagy. In H. polymorpha mig1mig2 double-deletion cells, macropexophagy was also substantially impaired, whereas micropexophagy became a dominant mode of autophagic degradation. Our findings suggest that homologues of the elements of the Saccharomyces cerevisiae main repression pathway have pleiotropic functions in H. polymorpha.

Originele taal-2English
Pagina's (van-tot)1103-1113
Aantal pagina's11
TijdschriftFems Yeast Research
Volume7
Nummer van het tijdschrift7
DOI's
StatusPublished - okt.-2007
Evenement4th Hansenula Polymorpha Worldwide Network Conference (HPWN) - , Netherlands
Duur: 3-sep.-20065-sep.-2006

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