Tissue integration versus bacterial colonization on dental implant materials

Bingran Zhao

Tissue integration versus bacterial colonization on dental implant materials

Bingran Zhao

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Biomaterialen worden in de huidige geneeskunde vaak als implantaat gebruikt om de functie en vorm van het menselijk lichaam te herstellen. Echter, de aanwezigheid van een biomateriaal vergroot de kans op infectie drastisch. Om infectie gerelateerd falen van implantaten te voorkomen hebben we verschillende materialen en oppervlakte modificaties toegepast die weefsel integratie verbeteren en biofilm formatie tegen gaan op tandheelkundige implantaten.
In het begin van dit proefschrift hebben we onderbouwd waarom tandheelkundige implantaten, die zich in een bacterie rijk milieu bevinden, zo vaak succesvol zijn, terwijl andere implantaten in een bijna steriele omgeving falen. De voornaamste reden is het snelle herstel van orale weefsels en de tolerantie van het orale immuun systeem. Om de orale omgeving na te bootsen werden twee modellen ontwikkeld, het peri-operatieve model, gebruikmakend van supra-gingivale bacteriële stammen versus humane gingivale fibroblasten, en een laat post-operatief model met periodontale pathogenen versus osteoblasten. De resultaten wijzen op verschillende materialen voor het stimuleren van de formatie van een afdichting van zacht weefsel gevormd door fibroblasten vergeleken met het behouden van integratie in het bot; de nek van het implantaat zou bij voorkeur van glad titanium gemaakt moeten worden, terwijl voor integratie in het bot een titanium-zirkonium legering en andere zirkonium varianten de voorkeur hebben. De gebruikte co-cultuur (bacteriën en cellen) studies in dit proefschrift zijn in lijn met klinische bevindingen en bieden relevantere informatie dan traditionele in vitro mono-cultuur studies die alleen de bacteriële of alleen weefsel respons op biomateriaal bekijken.

Originele taal-2	English
Kwalificatie	Doctor of Philosophy
Toekennende instantie	Rijksuniversiteit Groningen
Begeleider(s)/adviseur	Ren, Yijin, Supervisor Busscher, Henk, Supervisor van der Mei, Henny, Supervisor
Datum van toekenning	21-sep.-2015
Plaats van publicatie	[Groningen]
Uitgever	University of Groningen
Gedrukte ISBN's	978-90-367-8096-4
Elektronische ISBN's	978-90-367-8095-7
Status	Published - 2015

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title = "Tissue integration versus bacterial colonization on dental implant materials",

abstract = "Biomaterial implants are frequently used in modern medicine to restore function and form of human anatomy. However, the presence of implanted biomaterials dramatically elevates infection risk. In order to reduce the incidence of infection-associated implant failure, we have applied different materials and surface modifications for tissue integration improvement and inhibiting biofilm formation on dental implants. At the beginning of this thesis we identified causes as to why dental implants, which are in a bacterial-laden milieu, so often succeed where others, in a near-sterile environment, fail. The major reason is that oral tissues heal fast in the presence of bacteria, and tolerance of the oral immune-system. In order to mimic the oral environment, two in vitro models the peri-operative model, using supra-gingival bacterial strains versus human gingival fibroblast cells, and a late post-operative model involving periodontopathogens versus osteoblast cells have been developed. Results point to a different material for stimulating the formation of a soft tissue seal by the fibroblasts compared to the preservation of osseointegration; the neck of the implant should preferentially be made of smooth titanium, while for osseointegratable parts titanium-zirconium alloy and zirconia variants are to be preferred. The used co-culture studies in this thesis are in line with clinical findings and provide more relevant information than traditional in vitro mono-culture studies addressing solely bacterial or tissue response to biomaterials.",

author = "Bingran Zhao",

year = "2015",

language = "English",

isbn = "978-90-367-8096-4",

publisher = "University of Groningen",

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T1 - Tissue integration versus bacterial colonization on dental implant materials

AU - Zhao, Bingran

PY - 2015

Y1 - 2015

N2 - Biomaterial implants are frequently used in modern medicine to restore function and form of human anatomy. However, the presence of implanted biomaterials dramatically elevates infection risk. In order to reduce the incidence of infection-associated implant failure, we have applied different materials and surface modifications for tissue integration improvement and inhibiting biofilm formation on dental implants. At the beginning of this thesis we identified causes as to why dental implants, which are in a bacterial-laden milieu, so often succeed where others, in a near-sterile environment, fail. The major reason is that oral tissues heal fast in the presence of bacteria, and tolerance of the oral immune-system. In order to mimic the oral environment, two in vitro models the peri-operative model, using supra-gingival bacterial strains versus human gingival fibroblast cells, and a late post-operative model involving periodontopathogens versus osteoblast cells have been developed. Results point to a different material for stimulating the formation of a soft tissue seal by the fibroblasts compared to the preservation of osseointegration; the neck of the implant should preferentially be made of smooth titanium, while for osseointegratable parts titanium-zirconium alloy and zirconia variants are to be preferred. The used co-culture studies in this thesis are in line with clinical findings and provide more relevant information than traditional in vitro mono-culture studies addressing solely bacterial or tissue response to biomaterials.

AB - Biomaterial implants are frequently used in modern medicine to restore function and form of human anatomy. However, the presence of implanted biomaterials dramatically elevates infection risk. In order to reduce the incidence of infection-associated implant failure, we have applied different materials and surface modifications for tissue integration improvement and inhibiting biofilm formation on dental implants. At the beginning of this thesis we identified causes as to why dental implants, which are in a bacterial-laden milieu, so often succeed where others, in a near-sterile environment, fail. The major reason is that oral tissues heal fast in the presence of bacteria, and tolerance of the oral immune-system. In order to mimic the oral environment, two in vitro models the peri-operative model, using supra-gingival bacterial strains versus human gingival fibroblast cells, and a late post-operative model involving periodontopathogens versus osteoblast cells have been developed. Results point to a different material for stimulating the formation of a soft tissue seal by the fibroblasts compared to the preservation of osseointegration; the neck of the implant should preferentially be made of smooth titanium, while for osseointegratable parts titanium-zirconium alloy and zirconia variants are to be preferred. The used co-culture studies in this thesis are in line with clinical findings and provide more relevant information than traditional in vitro mono-culture studies addressing solely bacterial or tissue response to biomaterials.

M3 - Thesis fully internal (DIV)

SN - 978-90-367-8096-4

PB - University of Groningen

CY - [Groningen]

ER -

Tissue integration versus bacterial colonization on dental implant materials

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