Topologically fixed SecG is fully functional

Eli O. van der Sluis; E van der Vries; G Berrelkamp; N Nouwen; AJM Driessen

doi:10.1128/JB.188.3.1188-1190.2006

Topologically fixed SecG is fully functional

Eli O. van der Sluis, E van der Vries, G Berrelkamp, N Nouwen, AJM Driessen^*

^*Bijbehorende auteur voor dit werk

Onderzoeksoutput › Academic › peer review

18 Citaten (Scopus)

227 Downloads (Pure)

Samenvatting

It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

Originele taal-2	English
Pagina's (van-tot)	1188-1190
Aantal pagina's	3
Tijdschrift	Journal of Bacteriology
Volume	188
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1128/JB.188.3.1188-1190.2006
Status	Published - feb.-2006

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10.1128/JB.188.3.1188-1190.2006

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title = "Topologically fixed SecG is fully functional",

abstract = "It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.",

keywords = "PROTEIN TRANSLOCATION MACHINERY, ESCHERICHIA-COLI, CROSS-LINKING, CYTOPLASMIC MEMBRANE, PREPROTEIN, COMPLEX, INVERSION, COMPONENT, SUBUNITS, ROLES",

author = "Sluis, {Eli O. van der} and {van der Vries}, E and G Berrelkamp and N Nouwen and AJM Driessen",

year = "2006",

month = feb,

doi = "10.1128/JB.188.3.1188-1190.2006",

language = "English",

volume = "188",

pages = "1188--1190",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "AMER SOC MICROBIOLOGY",

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TY - JOUR

T1 - Topologically fixed SecG is fully functional

AU - Sluis, Eli O. van der

AU - van der Vries, E

AU - Berrelkamp, G

AU - Nouwen, N

AU - Driessen, AJM

PY - 2006/2

Y1 - 2006/2

N2 - It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

AB - It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

KW - PROTEIN TRANSLOCATION MACHINERY

KW - ESCHERICHIA-COLI

KW - CROSS-LINKING

KW - CYTOPLASMIC MEMBRANE

KW - PREPROTEIN

KW - COMPLEX

KW - INVERSION

KW - COMPONENT

KW - SUBUNITS

KW - ROLES

U2 - 10.1128/JB.188.3.1188-1190.2006

DO - 10.1128/JB.188.3.1188-1190.2006

M3 - Article

VL - 188

SP - 1188

EP - 1190

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 3

ER -