Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches

Susanne Wolff; Haike Antelmann; Dirk Albrecht; Doerte Becher; Joerg Bernhardt; Sierd Bron; Knut Buettner; Jan Maarten van Dijl; Christine Eymann; Andreas Otto; Le Thi Tam; Michael Hecker

doi:10.1016/j.jchromb.2006.09.029

Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches

Susanne Wolff, Haike Antelmann, Dirk Albrecht, Doerte Becher, Joerg Bernhardt, Sierd Bron, Knut Buettner, Jan Maarten van Dijl, Christine Eymann, Andreas Otto, Le Thi Tam, Michael Hecker^*

^*Corresponding author voor dit werk

Onderzoeksoutput › peer review

51 Citaten (Scopus)

Samenvatting

With the emergence of mass spectrometry in protein science and the availability of complete genome sequences, proteomics has gone through a rapid development. The soil bacterium Bacillus subtilis, as one of the first DNA sequenced species, represents a model for Gram-positive bacteria and its proteome was extensively studied throughout the years. Having the final goal to elucidate how life really functions, one basic requirement is to know the entirety of cellular proteins. This review presents how far we have got in unraveling the proteome of B. subtilis. The application of gel-based and gel-free technologies, the analyses of different subcellular proteome fractions, and the pursuance of various physiological strategies resulted in a coverage of more than one-third of B. subtilis theoretical proteome. (c) 2006 Elsevier B.V. All rights reserved.

Originele taal-2	English
Pagina's (van-tot)	129-140
Aantal pagina's	12
Tijdschrift	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume	849
Nummer van het tijdschrift	1-2
DOI's	https://doi.org/10.1016/j.jchromb.2006.09.029
Status	Published - 15-apr.-2007

Toegang tot document

10.1016/j.jchromb.2006.09.029

Handle.net

Permanente koppeling

Document onder embargo

Towards the entire proteome of the model bacterium Bacillus
Final publisher's version, 2,63 MB
Kopie aanvragen

Citeer dit

Wolff, S., Antelmann, H., Albrecht, D., Becher, D., Bernhardt, J., Bron, S., Buettner, K., van Dijl, J. M., Eymann, C., Otto, A., Tam, L. T., & Hecker, M. (2007). Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 849(1-2), 129-140. https://doi.org/10.1016/j.jchromb.2006.09.029

@article{471be710abfc4a20ace106f1b6a371bb,

title = "Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches",

abstract = "With the emergence of mass spectrometry in protein science and the availability of complete genome sequences, proteomics has gone through a rapid development. The soil bacterium Bacillus subtilis, as one of the first DNA sequenced species, represents a model for Gram-positive bacteria and its proteome was extensively studied throughout the years. Having the final goal to elucidate how life really functions, one basic requirement is to know the entirety of cellular proteins. This review presents how far we have got in unraveling the proteome of B. subtilis. The application of gel-based and gel-free technologies, the analyses of different subcellular proteome fractions, and the pursuance of various physiological strategies resulted in a coverage of more than one-third of B. subtilis theoretical proteome. (c) 2006 Elsevier B.V. All rights reserved.",

keywords = "proteome coverage, stress and starvation signatures, subproteomic fractions, gel-based and gel-free proteomics, STARVATION-INDUCIBLE PROTEINS, GRAM-POSITIVE BACTERIA, HEAT-SHOCK PROTEINS, MASS-SPECTROMETRY, 2-DIMENSIONAL ELECTROPHORESIS, IDENTIFICATION TECHNOLOGY, PHYSIOLOGICAL PROTEOMICS, TRANSCRIPTIONAL ANALYSIS, HALOBACTERIUM-SALINARUM, HAEMOPHILUS-INFLUENZAE",

author = "Susanne Wolff and Haike Antelmann and Dirk Albrecht and Doerte Becher and Joerg Bernhardt and Sierd Bron and Knut Buettner and {van Dijl}, {Jan Maarten} and Christine Eymann and Andreas Otto and Tam, {Le Thi} and Michael Hecker",

year = "2007",

month = apr,

day = "15",

doi = "10.1016/j.jchromb.2006.09.029",

language = "English",

volume = "849",

pages = "129--140",

journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "ELSEVIER SCIENCE BV",

number = "1-2",

}

Wolff, S, Antelmann, H, Albrecht, D, Becher, D, Bernhardt, J, Bron, S, Buettner, K, van Dijl, JM, Eymann, C, Otto, A, Tam, LT & Hecker, M 2007, 'Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches', Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, vol. 849, nr. 1-2, blz. 129-140. https://doi.org/10.1016/j.jchromb.2006.09.029

TY - JOUR

T1 - Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches

AU - Wolff, Susanne

AU - Antelmann, Haike

AU - Albrecht, Dirk

AU - Becher, Doerte

AU - Bernhardt, Joerg

AU - Bron, Sierd

AU - Buettner, Knut

AU - van Dijl, Jan Maarten

AU - Eymann, Christine

AU - Otto, Andreas

AU - Tam, Le Thi

AU - Hecker, Michael

PY - 2007/4/15

Y1 - 2007/4/15

N2 - With the emergence of mass spectrometry in protein science and the availability of complete genome sequences, proteomics has gone through a rapid development. The soil bacterium Bacillus subtilis, as one of the first DNA sequenced species, represents a model for Gram-positive bacteria and its proteome was extensively studied throughout the years. Having the final goal to elucidate how life really functions, one basic requirement is to know the entirety of cellular proteins. This review presents how far we have got in unraveling the proteome of B. subtilis. The application of gel-based and gel-free technologies, the analyses of different subcellular proteome fractions, and the pursuance of various physiological strategies resulted in a coverage of more than one-third of B. subtilis theoretical proteome. (c) 2006 Elsevier B.V. All rights reserved.

AB - With the emergence of mass spectrometry in protein science and the availability of complete genome sequences, proteomics has gone through a rapid development. The soil bacterium Bacillus subtilis, as one of the first DNA sequenced species, represents a model for Gram-positive bacteria and its proteome was extensively studied throughout the years. Having the final goal to elucidate how life really functions, one basic requirement is to know the entirety of cellular proteins. This review presents how far we have got in unraveling the proteome of B. subtilis. The application of gel-based and gel-free technologies, the analyses of different subcellular proteome fractions, and the pursuance of various physiological strategies resulted in a coverage of more than one-third of B. subtilis theoretical proteome. (c) 2006 Elsevier B.V. All rights reserved.

KW - proteome coverage

KW - stress and starvation signatures

KW - subproteomic fractions

KW - gel-based and gel-free proteomics

KW - STARVATION-INDUCIBLE PROTEINS

KW - GRAM-POSITIVE BACTERIA

KW - HEAT-SHOCK PROTEINS

KW - MASS-SPECTROMETRY

KW - 2-DIMENSIONAL ELECTROPHORESIS

KW - IDENTIFICATION TECHNOLOGY

KW - PHYSIOLOGICAL PROTEOMICS

KW - TRANSCRIPTIONAL ANALYSIS

KW - HALOBACTERIUM-SALINARUM

KW - HAEMOPHILUS-INFLUENZAE

U2 - 10.1016/j.jchromb.2006.09.029

DO - 10.1016/j.jchromb.2006.09.029

M3 - Review article

SN - 1570-0232

VL - 849

SP - 129

EP - 140

JO - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

IS - 1-2

ER -

Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches

Samenvatting

Toegang tot document

Handle.net

Document onder embargo

Vingerafdruk

Citeer dit