Tracing Dynamics and Clonal Heterogeneity of Cbx7-Induced Leukemic Stem Cells by Cellular Barcoding

Karin Klauke; Mathilde J. C. Broekhuis; Ellen Weersing; Albertina Dethmers-Ausema; Martha Ritsema; Marta Vila Gonzalez; Erik Zwart; Leonid V. Bystrykh; Gerald de Haan

doi:10.1016/j.stemcr.2014.10.012

Tracing Dynamics and Clonal Heterogeneity of Cbx7-Induced Leukemic Stem Cells by Cellular Barcoding

Karin Klauke^*, Mathilde J. C. Broekhuis, Ellen Weersing, Albertina Dethmers-Ausema, Martha Ritsema, Marta Vila Gonzalez, Erik Zwart, Leonid V. Bystrykh, Gerald de Haan

^*Bijbehorende auteur voor dit werk

Stem Cell Aging Leukemia and Lymphoma (SALL)

Onderzoeksoutput › Academic › peer review

10 Citaten (Scopus)

238 Downloads (Pure)

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Accurate monitoring of tumor dynamics and leukemic stem cell (LSC) heterogeneity is important for the development of personalized cancer therapies. In this study, we experimentally induced distinct types of leukemia in mice by enforced expression of Cbx7. Simultaneous cellular barcoding allowed for thorough analysis of leukemias at the clonal level and revealed high and unpredictable tumor complexity. Multiple LSC clones with distinct leukemic properties coexisted. Some of these clones remained dormant but bore leukemic potential, as they progressed to full-blown leukemia after challenge. LSC clones could retain multilineage differentiation capacities, where one clone induced phenotypically distinct leukemias. Beyond a detailed insight into CBX7-driven leukemic biology, our model is of general relevance for the understanding of tumor dynamics and clonal evolution.

Originele taal-2	English
Pagina's (van-tot)	74-89
Aantal pagina's	16
Tijdschrift	Stem Cell Reports
Volume	4
Nummer van het tijdschrift	1
DOI's	https://doi.org/10.1016/j.stemcr.2014.10.012
Status	Published - 13-jan-2015

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10.1016/j.stemcr.2014.10.012Licentie: CC BY-NC-ND

Tracing Dynamics and Clonal Heterogeneity of Cbx7-Induced Leukemic Stem Cells by Cellular BarcodingFinal publisher's version, 1,58 MBLicentie: CC BY-NC-ND

Citeer dit

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title = "Tracing Dynamics and Clonal Heterogeneity of Cbx7-Induced Leukemic Stem Cells by Cellular Barcoding",

abstract = "Accurate monitoring of tumor dynamics and leukemic stem cell (LSC) heterogeneity is important for the development of personalized cancer therapies. In this study, we experimentally induced distinct types of leukemia in mice by enforced expression of Cbx7. Simultaneous cellular barcoding allowed for thorough analysis of leukemias at the clonal level and revealed high and unpredictable tumor complexity. Multiple LSC clones with distinct leukemic properties coexisted. Some of these clones remained dormant but bore leukemic potential, as they progressed to full-blown leukemia after challenge. LSC clones could retain multilineage differentiation capacities, where one clone induced phenotypically distinct leukemias. Beyond a detailed insight into CBX7-driven leukemic biology, our model is of general relevance for the understanding of tumor dynamics and clonal evolution.",

keywords = "ACUTE MYELOID-LEUKEMIA, ACUTE LYMPHOBLASTIC-LEUKEMIA, CHRONIC LYMPHOCYTIC-LEUKEMIA, LINEAGE SWITCH, SELF-RENEWAL, EVOLUTION, MUTATIONS, HIERARCHY, PATTERNS, RELAPSE",

author = "Karin Klauke and Broekhuis, {Mathilde J. C.} and Ellen Weersing and Albertina Dethmers-Ausema and Martha Ritsema and Gonzalez, {Marta Vila} and Erik Zwart and Bystrykh, {Leonid V.} and {de Haan}, Gerald",

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Tracing Dynamics and Clonal Heterogeneity of Cbx7-Induced Leukemic Stem Cells by Cellular Barcoding. / Klauke, Karin; Broekhuis, Mathilde J. C.; Weersing, Ellen ; Dethmers-Ausema, Albertina; Ritsema, Martha; Gonzalez, Marta Vila; Zwart, Erik ; Bystrykh, Leonid V.; de Haan, Gerald.

In: Stem Cell Reports, Vol. 4, Nr. 1, 13.01.2015, blz. 74-89.

Onderzoeksoutput › Academic › peer review

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AU - Klauke, Karin

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AU - Weersing, Ellen

AU - Dethmers-Ausema, Albertina

AU - Ritsema, Martha

AU - Gonzalez, Marta Vila

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AU - Bystrykh, Leonid V.

AU - de Haan, Gerald

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AB - Accurate monitoring of tumor dynamics and leukemic stem cell (LSC) heterogeneity is important for the development of personalized cancer therapies. In this study, we experimentally induced distinct types of leukemia in mice by enforced expression of Cbx7. Simultaneous cellular barcoding allowed for thorough analysis of leukemias at the clonal level and revealed high and unpredictable tumor complexity. Multiple LSC clones with distinct leukemic properties coexisted. Some of these clones remained dormant but bore leukemic potential, as they progressed to full-blown leukemia after challenge. LSC clones could retain multilineage differentiation capacities, where one clone induced phenotypically distinct leukemias. Beyond a detailed insight into CBX7-driven leukemic biology, our model is of general relevance for the understanding of tumor dynamics and clonal evolution.

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