Transcriptional modulations induced by proton irradiation in mice skin in function of adsorbed dose and distance

Valerio Licursi, Wei Wang, Elena Di Nisio, Francesco P. Cammarata, Rosaria Acquaviva, Giorgio Russo, Lorenzo Manti, Mariangela Cestelli Guidi*, Emiliano Fratini, Gihan Kamel, Roberto Amendola, Pietro Pisciotta, Rodolfo Negri

*Bijbehorende auteur voor dit werk

    OnderzoeksoutputAcademicpeer review

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    Hadron therapy by proton beams represents an advanced anti-cancer strategy due to its highly localized dose deposition allowing a greater sparing of normal tissue and/or organs at risk compared to photon/electron radiotherapy. However, it is not clear to what extent non-targeted effects such as transcriptional modulations produced along the beamline may diffuse and impact the surrounding tissue. In this work, we analyze the transcriptome of proton-irradiated mouse skin and choose two biomarker genes to trace their modulation at different distances from the beam's target and at different doses and times from irradiation to understand to what extent and how far it may propagate, using RNA-Seq and quantitative RT-PCR. In parallel, assessment of lipids alteration is performed by FTIR spectroscopy as a measure of tissue damage. Despite the observed high individual variability of expression, we can show evidence of transcriptional modulation of two biomarker genes at considerable distance from the beam's target where a simulation system predicts a significantly lower adsorbed dose. The results are compatible with a model involving diffusion of transcripts or regulatory molecules from high dose irradiated cells to distant tissue's portions adsorbing a much lower fraction of radiation.

    Originele taal-2English
    Pagina's (van-tot)260-270
    Aantal pagina's11
    TijdschriftJournal of radiation research and applied sciences
    Nummer van het tijdschrift1
    Vroegere onlinedatum23-aug.-2021
    StatusPublished - 2021

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