tRNA separation by high-performance liquid chromatography using an aggregate of ODS-Hypersil and trioctylmethylammonium chloride

Rainer Bischoff, E. Graeser, L.W. Mclaughlin

OnderzoeksoutputAcademicpeer review

Samenvatting

High-performance liquid chromatography on a reversed-phase support treated with a tetraalkylammonium salt was used to separate tRNAs from baker's yeast. While resolution by this column appears to result from both anion-exchange and reversed-phase chromatography, it is the hydrophobic interactions which govern the separation of one tRNA from another. Chromatography of bulk tRNA resulted in a number of fractions with different amino acid acceptor activities and little cross-contamination. In some cases the column resolved several single nucleotide modifications of tRNAPhe. Using a 250 x 6.2 mm column it has been possible to chromatograph a minimum of 100 A260 units of tRNA without serious loss in resolution. tRNAs isolated from this column as the last step of a purification procedure have very high amino acid acceptor activities
Originele taal-2Dutch
Pagina's (van-tot)305 - 315
TijdschriftJ.Chromatogr.
Volume257
Nummer van het tijdschrift2
StatusPublished - 1983

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