Type VII Collagen in the Human Accommodation System: Expression in Ciliary Body, Zonules, and Lens Capsule

Bart Wullink; Hendri H. Pas; Roelofje J. Van der Worp; Martin Schol; Sarah F. Janssen; Roel Kuijer; Leonoor I. Los

doi:10.1167/iovs.17-23425

Type VII Collagen in the Human Accommodation System: Expression in Ciliary Body, Zonules, and Lens Capsule

Bart Wullink^*, Hendri H. Pas, Roelofje J. Van der Worp, Martin Schol, Sarah F. Janssen, Roel Kuijer, Leonoor I. Los

^*Bijbehorende auteur voor dit werk

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PURPOSE. To investigate intraocular expression of COL7A1 and its protein product type VII collagen, particularly at the accommodation system.

METHODS. Eyes from 26 human adult donors were used. COL7A1 expression was analyzed in ex vivo ciliary epithelium by microarray. Type VII collagen distribution was examined by Western blot analysis, immunohistochemistry. and immuno-electron microscopy.

RESULTS. COL7A1 is expressed by pigmented and nonpigmented ciliary epithelia. Type VII collagen is distributed particularly at the strained parts of the accommodation system. Type VII collagen was associated with various basement membranes and with ciliary zonules. Anchoring fibrils were not visualized.

CONCLUSIONS. Type VII collagen distribution at strained areas suggests a supporting role in tissue integrity.

Originele taal-2	English
Pagina's (van-tot)	1075-1083
Aantal pagina's	9
Tijdschrift	Investigative ophthalmology & visual science
Volume	59
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1167/iovs.17-23425
Status	Published - feb.-2018

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@article{0eaaba14c8214c0a939ee944d9fd508e,

title = "Type VII Collagen in the Human Accommodation System: Expression in Ciliary Body, Zonules, and Lens Capsule",

abstract = "PURPOSE. To investigate intraocular expression of COL7A1 and its protein product type VII collagen, particularly at the accommodation system.METHODS. Eyes from 26 human adult donors were used. COL7A1 expression was analyzed in ex vivo ciliary epithelium by microarray. Type VII collagen distribution was examined by Western blot analysis, immunohistochemistry. and immuno-electron microscopy.RESULTS. COL7A1 is expressed by pigmented and nonpigmented ciliary epithelia. Type VII collagen is distributed particularly at the strained parts of the accommodation system. Type VII collagen was associated with various basement membranes and with ciliary zonules. Anchoring fibrils were not visualized.CONCLUSIONS. Type VII collagen distribution at strained areas suggests a supporting role in tissue integrity.",

keywords = "collagen type VII, inner limiting membrane, basement membrane, accommodation, lens capsule, DERMAL-EPIDERMAL JUNCTION, ANCHORING FIBRILS, EPIDERMOLYSIS-BULLOSA, STRUCTURAL ORGANIZATION, PROTEOMIC ANALYSIS, EPITHELIUM, INSERTION, NETWORK, FORM, SKIN",

author = "Bart Wullink and Pas, {Hendri H.} and {Van der Worp}, {Roelofje J.} and Martin Schol and Janssen, {Sarah F.} and Roel Kuijer and Los, {Leonoor I.}",

year = "2018",

month = feb,

doi = "10.1167/iovs.17-23425",

language = "English",

volume = "59",

pages = "1075--1083",

journal = "Investigative ophthalmology & visual science",

issn = "0146-0404",

publisher = "ASSOC RESEARCH VISION OPHTHALMOLOGY INC",

number = "2",

}

TY - JOUR

T1 - Type VII Collagen in the Human Accommodation System

T2 - Expression in Ciliary Body, Zonules, and Lens Capsule

AU - Wullink, Bart

AU - Pas, Hendri H.

AU - Van der Worp, Roelofje J.

AU - Schol, Martin

AU - Janssen, Sarah F.

AU - Kuijer, Roel

AU - Los, Leonoor I.

PY - 2018/2

Y1 - 2018/2

N2 - PURPOSE. To investigate intraocular expression of COL7A1 and its protein product type VII collagen, particularly at the accommodation system.METHODS. Eyes from 26 human adult donors were used. COL7A1 expression was analyzed in ex vivo ciliary epithelium by microarray. Type VII collagen distribution was examined by Western blot analysis, immunohistochemistry. and immuno-electron microscopy.RESULTS. COL7A1 is expressed by pigmented and nonpigmented ciliary epithelia. Type VII collagen is distributed particularly at the strained parts of the accommodation system. Type VII collagen was associated with various basement membranes and with ciliary zonules. Anchoring fibrils were not visualized.CONCLUSIONS. Type VII collagen distribution at strained areas suggests a supporting role in tissue integrity.

AB - PURPOSE. To investigate intraocular expression of COL7A1 and its protein product type VII collagen, particularly at the accommodation system.METHODS. Eyes from 26 human adult donors were used. COL7A1 expression was analyzed in ex vivo ciliary epithelium by microarray. Type VII collagen distribution was examined by Western blot analysis, immunohistochemistry. and immuno-electron microscopy.RESULTS. COL7A1 is expressed by pigmented and nonpigmented ciliary epithelia. Type VII collagen is distributed particularly at the strained parts of the accommodation system. Type VII collagen was associated with various basement membranes and with ciliary zonules. Anchoring fibrils were not visualized.CONCLUSIONS. Type VII collagen distribution at strained areas suggests a supporting role in tissue integrity.

KW - collagen type VII

KW - inner limiting membrane

KW - basement membrane

KW - accommodation

KW - lens capsule

KW - DERMAL-EPIDERMAL JUNCTION

KW - ANCHORING FIBRILS

KW - EPIDERMOLYSIS-BULLOSA

KW - STRUCTURAL ORGANIZATION

KW - PROTEOMIC ANALYSIS

KW - EPITHELIUM

KW - INSERTION

KW - NETWORK

KW - FORM

KW - SKIN

U2 - 10.1167/iovs.17-23425

DO - 10.1167/iovs.17-23425

M3 - Article

C2 - 29490344

SN - 0146-0404

VL - 59

SP - 1075

EP - 1083

JO - Investigative ophthalmology & visual science

JF - Investigative ophthalmology & visual science

IS - 2

ER -