Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone

Katarzyna Tych*, Matthias Rief

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

Samenvatting

The heat shock protein 90 (Hsp90) family of chaperones are well-known, highly important components of the cellular systems which regulate protein homeostasis. Essential in eukaryotes, Hsp90s is also found in prokaryotes, including archaea. Hsp90 is a dimeric protein, with each monomer consisting of three separate structural domains, and undergoes large conformational changes as part of its functional cycle. This cycle is driven by interactions with nucleotides, cochaperone proteins, client proteins and allosteric effects enacted by these and by posttranslational modifications. All of these influence the rate and degree of the opening and closing of the dimer as well as the relative domain orientations and its overall rigidity. Optical tweezers, which can access many of these functionally important conformational changes, therefore provide a unique tool for the study of this large and complex molecular chaperone. Here, we provide protocols for the design and implementation of different Hsp90 constructs and optical tweezers experiments for addressing the many open questions about the function of this important molecular chaperone.

Originele taal-2English
TitelOptical Tweezers
UitgeverijHumana, New York, NY
Pagina's401-425
Aantal pagina's25
Volume2478
ISBN van elektronische versie978-1-0716-2229-2
ISBN van geprinte versie978-1-0716-2228-5
DOI's
StatusPublished - 6-sep-2022

Publicatie series

NaamMethods in molecular biology (Clifton, N.J.)
ISSN van geprinte versie1064-3745

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