Validated liquid chromatographic method for the determination of bexarotene in human plasma

N.C. van de Merbel, J H van Veen, G Wilkens, G Loewen

OnderzoeksoutputAcademicpeer review

14 Citaten (Scopus)


A new liquid chromatographic method is described for the determination of the anti-tumour agent bexarotene in human plasma over the range 0.500-1500 ng/ml, using 1 ml of sample. Sample preparation consists of liberating the analyte from plasma lipids by adding acetonitrile, followed by acidification of the plasma and liquid extraction using a mixture of isoamyl alcohol and pentane or hexane. Separation and quantitation are performed by reversed-phase column liquid chromatography with fluorescence detection. Parameters affecting the performance of these steps are discussed. Validation results on linearity, selectivity, accuracy, precision, recovery and stability are shown, as well as the application of the method to samples from clinical trials.

Originele taal-2English
Pagina's (van-tot)189-95
Aantal pagina's7
TijdschriftJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Nummer van het tijdschrift2
StatusPublished - 5-aug.-2002
Extern gepubliceerdJa

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