Visualizing Molecular Dynamics by High-Speed Atomic Force Microscopy

Chris van Ewijk, Sourav Maity, Wouter H Roos*

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review


Dynamic processes and structural changes of biological molecules are essential to life. While conventional atomic force microscopy (AFM) is able to visualize molecules and supramolecular assemblies at sub-nanometer resolution, it cannot capture dynamics because of its low imaging rate. The introduction of high-speed atomic force microscopy (HS-AFM) solved this problem by providing a large increase in imaging velocity. Using HS-AFM, one is able to visualize dynamic molecular events with high spatiotemporal resolution under near-to physiological conditions. This approach opened new windows as finally dynamics of biomolecules at sub-nanometer resolution could be studied. Here we describe the working principles and an operation protocol for HS-AFM imaging and characterization of biological samples in liquid.

Originele taal-2English
TitelSingle Molecule Analysis
RedacteurenI. Heller, D. Dulin, E.J. Peterman
UitgeverijHumana, New York, NY
Aantal pagina's18
StatusPublished - 2024

Publicatie series

NaamMethods in molecular biology (Clifton, N.J.)
UitgeverijHumana, New York, NY
ISSN van geprinte versie1064-3745

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