Weight shapes the intestinal microbiome in preterm infants: results of a prospective observational study

Fardou H. Heida*, Elisabeth M. W. Kooi, Josef Wagner, Thi-Yen Nguyen, Jan B. F. Hulscher, Anne G. J. F. van Zoonen, Arend F. Bos, Hermie J. M. Harmsen, Marcus C. de Goffau

*Bijbehorende auteur voor dit werk

OnderzoeksoutputAcademicpeer review

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Background The intestinal microbiome in preterm infants differs markedly from term infants. It is unclear whether the microbiome develops over time according to infant specific factors. Methods We analysed (clinical) metadata - to identify the main factors influencing the microbiome composition development - and the first meconium and faecal samples til the 4th week via 16 S rRNA amplican sequencing. Results We included 41 infants (gestational age 25-30 weeks; birth weight 430-990 g. Birth via Caesarean section (CS) was associated with placental insufficiency during pregnancy and lower BW. In meconium samples and in samples from weeks 2 and 3 the abundance of Escherichia and Bacteroides (maternal faecal representatives) were associated with vaginal delivery while Staphylococcus (skin microbiome representative) was associated with CS. Secondly, irrespective of the week of sampling or the mode of birth, a transition was observed as children children gradually increased in weight from a microbiome dominated by Staphylococcus (Bacilli) towards a microbiome dominated by Enterobacteriaceae (Gammaproteobacteria). Conclusions Our data show that the mode of delivery affects the meconium microbiome composition. They also suggest that the weight of the infant at the time of sampling is a better predictor for the stage of progression of the intestinal microbiome development/maturation than postconceptional age as it less confounded by various infant-specific factors.

Originele taal-2English
Artikelnummer219
Pagina's (van-tot)1-12
Aantal pagina's12
TijdschriftBMC Microbiology
Volume21
Nummer van het tijdschrift1
DOI's
StatusPublished - 21-jul-2021

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