Westernized high-fat diet accelerates weight loss in dextran sulfate sodium-induced colitis in mice, which is further aggravated by supplementation of heme

The Western diet, rich in fat and red meat, predisposes for inflammatory bowel disease (IBD); however, little is known about mechanisms involved. Red meat contains high levels of heme, a well-known inducer of the cytoprotective enzyme heme oxygenase-1 (HO-1). Pharmacological induction of HO-1 ameliorates experimental colitis. We analyzed the effect of a westernized high-fat (HF) diet supplemented with heme on intestinal HO-1 expression and dextran sulfate sodium (DSS)-induced colitis.

Mice were fed chow or HF diets for 2 weeks. In the second week, the HF diet was supplemented with or without 0.5 mu mol/g heme. Subsequently, the 3 diet groups were given drinking water with or without 4% DSS to induce colitis.

Significant body weight reduction was first observed after 4 days in the chow/DSS mice (-5 +/- 3%), whereas this was evident already after 2 days (-6 +/- 2%) in HF/DSS mice, showing increased weight loss compared to chow/DSS mice in the following days. Heme supplementation further aggravated DSS-induced weight loss in HF mice (-18 +/- 4% vs. -7 +/- 5% for HF+heme/DSS vs. HF/DSS, P

A westernized diet accelerates DSS-induced weight loss in mice, which is further aggravated by heme, despite the induction of HO-1 in the colon epithelium. Our data warrant a detailed analysis of the association of (red) meat-containing diets and the development of IBD. (C) 2013 Elsevier Inc. All rights reserved.